MolMarker: A Simple Tool for DNA Fingerprinting Studies and Polymorphic Information Content Calculation

Molecular markers and mapping are used to analyze an organism’s genes. They allow the selection of target genetic areas based on marker genotype (and not trait phenotype), facilitate study variability diversity, create linkage maps, follow individuals or lines carrying certain may be select parental genotypes, remove drag in back-crossing, choose difficult-to-measure characteristics. Due a lack variety crops, gene pools wild crop relatives for future agricultural production have been examined. The invention RFLP (Restriction Fragment Length Polymorphism) allowed creation other traditional approaches such as RAPD (Random Amplified Polymorphic DNA) AFLP (Amplified Polymorphism). Accordingly, need describe polymorphic information content (PIC) ideal has raised. Marker reliability depends marker’s relationship genomic area interest. Although informativeness must estimated design, there no readily available tools. Earlier, PICcalc was developed calculate heterozygosity (H) PIC simplify molecular investigations. These two values were corrected dominant co-dominant (binary allelic data) determine polymorphism quality. popularity web, we downloadable version called MolMarker with extra functionality reduce server maintenance.